6.5% NaCl Broth


What is this medium used for?


Growth of some bacteria is inhibited by sodium chloride (NaCl), commonly known as table salt. Others are capable of growing in its presence. This medium allows determination of which category an unknown bacterium falls into.



How is growth in 6.5% NaCl determined? 


If a microbe is sensitive to NaCl, it will be unable to grow in 6.5% NaCl broth. After 24 hours incubation, there would be no turbidity (cloudiness) in the broth, indicating no growth had occurred. If a microbe CAN grow in 6.5% NaCl broth, the medium will be turbid at 24 hours.


What is the content of this medium?  


6.5% NaCl broth is a nutrient broth to which 6.5% NaCl has been added.



How is the test performed?  


For information on how to determine the ability of a microbe to grow in the presence of 6.5% NaCl broth, refer to the growth on 6.5% NaCl test.